Anthers collected from 8 grape cultivars (Black Hamburg, Riesling, Campbell Early, Steuben, Kyoho, Delaware, Early Niabell, Muscat Bailey-A) were cultured aseptically on modified Murashige and Skoog´s medium, and their responses in callus formation, multiplication, and organogenesis were studied.
1. Induced calli from grape anthers were creamy, and the color was milk white except ´Delaware´ when grown on a modified Murashige and Skoog´s medium.
2. Varietal behavior in callus formation was very different, ranging from 46.6% in ´Delaware´ to only 3.2% in ´Early Niabell´.
3. As maximum callus formation occurred in cultures grown on Murathige and Skoog´s medium with 2㎎/ℓ kinetin+0.5㎎/ℓ IAA+0.5㎎/ℓ NAA, NAA seems to be more effective than IAA or IBA when the concentration of kinetin is fixed.
4. No organs developed on the media for regeneration as in other woody species.
|